pYTK091

65198 Synthetic Bacterial Expression

Plasmid Information

Purpose
Encodes SpecR-ColE1 as a Type 8a part to be used in the Dueber YTK system
Depositor
John Dueber
Article
Lee et al ACS Synth Biol. 2015 Apr 14.
Insert
SpecR-ColE1
Available Since
June 16, 2015
Availability
Available

Sequence Information

Length
779 bp
Topology
linear
Molecule Type
genomic DNA

Features

terminator 2
Label Gene Product Position Strand
T7Te terminator 105..133 Rev
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
rrnB T1 terminator Escherichia coli rrnB 148..220 Rev
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC