pYTK060

65167 Synthetic Bacterial Expression

Plasmid Information

Purpose
Encodes 6XHis_3XFlag as a Type 4a part to be used in the Dueber YTK system
Depositor
John Dueber
Article
Lee et al ACS Synth Biol. 2015 Apr 14.
Insert
6XHis_3XFlag
Available Since
June 16, 2015
Availability
Available

Sequence Information

Length
836 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 153..171 Rev
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
3xFLAG three tandem FLAG(R) epitope tags, foll… 174..246 Rev
DNA Sequence
GATTACAAGGACGATGATGATAAGGACTACAAAGATGATGATGACAAAGATTATAAGGACGATGATGATAAA
Protein Sequence
DYKDDDDKDYKDDDDKDYKDDDDK
terminator 1
Label Gene Product Position Strand
lambda t0 terminator 92..127 Fwd
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT