pYTK046

65153 Synthetic Bacterial Expression

Plasmid Information

Purpose
Encodes mRuby2 as a Type 3b part to be used in the Dueber YTK system
Depositor
John Dueber
Article
Lee et al ACS Synth Biol. 2015 Apr 14.
Insert
mRuby2
Available Since
June 16, 2015
Availability
Available

Sequence Information

Length
1,038 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
mRuby2 — monomeric red fluorescent protein deriv… 135..843 Fwd
DNA Sequence
GTGTCCAAAGGAGAGGAGTTAATCAAGGAAAACATGAGAATGAAAGTTGTCATGGAGGGCTCCGTTAATGGTCACCAATTCAAGTGTACAGGGGAAGGTGAAGGTAATCCTTACATGGGTACACAAACTATGAGAATTAAAGTAATTGAAGGCGGACCACTACCATTTGCATTTGACATTCTGGCAACGTCATTCATGTACGGATCACGAACTTTCATCAAGTACCCTAAAGGTATACCAGACTTTTTCAAGCAATCTTTTCCAGAGGGTTTTACATGGGAAAGGGTTACAAGATACGAAGATGGGGGTGTCGTCACAGTTATGCAAGATACTTCATTAGAAGATGGCTGCCTTGTCTATCATGTGCAAGTAAGAGGGGTGAATTTTCCTTCTAACGGACCTGTGATGCAGAAAAAGACCAAAGGTTGGGAACCAAATACTGAAATGATGTACCCAGCTGATGGAGGTTTGAGAGGCTACACACACATGGCGCTTAAAGTTGATGGTGGAGGTCATTTGTCTTGTAGTTTTGTTACCACTTATCGTTCTAAAAAGACTGTTGGCAATATCAAAATGCCAGGAATACATGCTGTAGACCACAGACTAGAAAGACTCGAAGAGAGCGATAACGAAATGTTCGTTGTACAGAGAGAGCATGCCGTAGCCAAATTTGCTGGCTTAGGCGGTGGTATGGATGAATTGTATAAG
Protein Sequence
VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMRIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHMALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFAGLGGGMDELYK
terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 862..897 Rev
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT