pYTK040

65147 Synthetic Bacterial Expression

Plasmid Information

Purpose
Encodes 6XHis_3XFlag as a Type 3a part to be used in the Dueber YTK system
Depositor
John Dueber
Article
Lee et al ACS Synth Biol. 2015 Apr 14.
Insert
6XHis_3XFlag
Available Since
June 16, 2015
Availability
Available

Sequence Information

Length
1,003 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 157..175 Rev
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
3xFLAG — three tandem FLAG(R) epitope tags, foll… 178..250 Rev
DNA Sequence
GATTACAAGGACGATGATGATAAGGACTACAAAGATGATGATGACAAAGATTATAAGGACGATGATGATAAA
Protein Sequence
DYKDDDDKDYKDDDDKDYKDDDDK
rep_origin 1
Label Gene Product Position Strand
ori — — 364..953 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 103..138 Fwd
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT