pYTK039

65146 Synthetic Bacterial Expression

Plasmid Information

Purpose
Encodes mRuby2 as a Type 3a part to be used in the Dueber YTK system
Depositor
John Dueber
Article
Lee et al ACS Synth Biol. 2015 Apr 14.
Insert
mRuby2
Available Since
June 16, 2015
Availability
Available

Sequence Information

Length
942 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
mRuby2 — monomeric red fluorescent protein deriv… 154..865 Rev
DNA Sequence
ATGGTGTCCAAAGGAGAGGAGTTAATCAAGGAAAACATGAGAATGAAAGTTGTCATGGAGGGCTCCGTTAATGGTCACCAATTCAAGTGTACAGGGGAAGGTGAAGGTAATCCTTACATGGGTACACAAACTATGAGAATTAAAGTAATTGAAGGCGGACCACTACCATTTGCATTTGACATTCTGGCAACGTCATTCATGTACGGATCACGAACTTTCATCAAGTACCCTAAAGGTATACCAGACTTTTTCAAGCAATCTTTTCCAGAGGGTTTTACATGGGAAAGGGTTACAAGATACGAAGATGGGGGTGTCGTCACAGTTATGCAAGATACTTCATTAGAAGATGGCTGCCTTGTCTATCATGTGCAAGTAAGAGGGGTGAATTTTCCTTCTAACGGACCTGTGATGCAGAAAAAGACCAAAGGTTGGGAACCAAATACTGAAATGATGTACCCAGCTGATGGAGGTTTGAGAGGCTACACACACATGGCGCTTAAAGTTGATGGTGGAGGTCATTTGTCTTGTAGTTTTGTTACCACTTATCGTTCTAAAAAGACTGTTGGCAATATCAAAATGCCAGGAATACATGCTGTAGACCACAGACTAGAAAGACTCGAAGAGAGCGATAACGAAATGTTCGTTGTACAGAGAGAGCATGCCGTAGCCAAATTTGCTGGCTTAGGCGGTGGTATGGATGAATTGTATAAG
Protein Sequence
MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMRIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHMALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFAGLGGGMDELYK
terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 100..135 Fwd
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT