pYTK030

65137 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Purpose
Encodes pGAL1 as a Type 2 part to be used in the Dueber YTK system
Depositor
John Dueber
Article
Lee et al ACS Synth Biol. 2015 Apr 14.
Insert
pGAL1
Available Since
June 16, 2015
Availability
Available

Sequence Information

Length
1,069 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
GAL1 promoter S. cerevisiae GAL1 — 215..657 Fwd
DNA Sequence
CGGATTAGAAGCCGCCGAGCGGGCGACAGCCCTCCGACGGATGACTCTCCTCCGTGCGTCCTCGTCATCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATTAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGGAAAAGCTGCATAACCACTTTAACTAATACTTTCAACATTTTCAGTTTGTATTACTTCTTATTCAAATGTCATAAAAGTATCAACAAAAAATTGTTAATATACCTCTATACTTTAACGTCAAGGAG
protein_bind 1
Label Gene Product Position Strand
UAS — — 215..333 Fwd
DNA Sequence
CGGATTAGAAGCCGCCGAGCGGGCGACAGCCCTCCGACGGATGACTCTCCTCCGTGCGTCCTCGTCATCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCG
terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 690..725 Rev
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT