pYTK013

65120 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Purpose
Encodes pTEF1 as a Type 2 part to be used in the Dueber YTK system
Depositor
John Dueber
Article
Lee et al ACS Synth Biol. 2015 Apr 14.
Insert
pTEF1
Available Since
June 16, 2015
Availability
Available

Sequence Information

Length
856 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
TEF1 promoter S. cerevisiae TEF1 — 174..588 Rev
DNA Sequence
GATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAG
terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 104..139 Fwd
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT