pBAD-hsGFP

64911 Synthetic Bacterial Expression

Plasmid Information

Purpose
Encodes a Fluorescent Reporter for Hydrogen Sulfide in E. Coli
Depositor
Huiwang Ai
Article
Chen et al Biochemistry. 2014 Sep 23;53(37):5966-74
Insert
hsGFP
Tags
His Tag
Mutation
Chromophore tyrosine of the protein mutated to TA…
Promoter
araBAD
Available Since
May 20, 2015
Availability
Available

Sequence Information

Length
803 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 0..18 Rev
DNA Sequence
CACCATCACCATCACCAT
Protein Sequence
HHHHHH