POLN delP_pDEST17

64886 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of human POLN with a short truncation of the C-terminal proline-rich tail
Depositor
Richard Wood
Article
Takata et al J Biol Chem. 2006 Aug 18;281(33):23445-5
Insert
POLN (POLN Human)
Tags
Flag and His
Mutation
with a short truncation of the C-terminal proline…
Promoter
T7
Available Since
July 11, 2016
Availability
Available

Sequence Information

Length
702 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 132..150 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 40..59 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
attB1 mutant version of attB — 159..184 Fwd
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT