POLN_pDEST17

64885 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of POLN for purification
Depositor
Richard Wood
Article
Takata et al Nucleic Acids Res. 2010 Jun;38(10):3233-
Insert
POLN (POLN Human)
Tags
Flag and His
Promoter
T7
Available Since
May 21, 2015
Availability
Available

Sequence Information

Length
819 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 144..162 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
HRV 3C site — recognition and cleavage site for human… 216..240 Fwd
DNA Sequence
CTGGAAGTTCTGTTCCAGGGGCCC
Protein Sequence
LEVLFQGP
promoter 1
Label Gene Product Position Strand
T7 promoter — — 52..71 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
attB1 mutant version of attB — 171..196 Fwd
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT