pRSET-A2-77p-Venus

64856 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tag
Depositor
Sriram Neelamegham
Article
Gogia et al PLoS One. 2015 May 18;10(5):e0126556. do
Insert
VWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Human, Synthetic)
Tags
7X His and Venus
Promoter
T7 Promoter
Available Since
May 8, 2015
Availability
Available

Sequence Information

Length
687 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
TEV site — tobacco etch virus (TEV) protease recog… 253..274 Fwd
DNA Sequence
GAGAATCTATACTTTCAAGGC
Protein Sequence
ENLYFQG