gRNA-his-HYB

64333 Synthetic Yeast Expression

Plasmid Information

Purpose
his3 deletion gRNA cassette carried by pRS42H
Depositor
Yong-Su Jin
Article
Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):
Insert
gBlock product of his3 deletion gRNA cassette
Available Since
April 29, 2015
Availability
Available

Sequence Information

Length
964 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 4
Label Gene Product Position Strand
lac promoter — — 130..161 Fwd
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
T3 promoter — — 229..248 Fwd
DNA Sequence
AATTAACCCTCACTAAAGG
SNR52 promoter — — 266..535 Fwd
DNA Sequence
TCTTTGAAAAGATAATGTATGATTATGCTTTCACTCATATTTATACAGAAACTTGATGTTTTCTTTCGAGTATATACAAGGTGATTACATGTACGTTTGAAGTACAACTCTAGATTTTGTAGTGCCCTCTTGGGCTAGCGGTAAAGGTGCGCATTTTTTCACACCCTACAATGTTCTGTTCAAAAGATTTTGGTCAAACGCTGTAGAAGTGAAAGTTGGTGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATC
T7 promoter — — 667..686 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 2
Label Gene Product Position Strand
CAP binding site — — 94..116 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator — — 168..185 Fwd
DNA Sequence
TTGTGAGCGGATAACAA