gRNA-leu-HYB — PlasmidDB

Plasmid Information

Purpose

leu2 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of leu2 deletion gRNA cassette

Available Since

April 29, 2015

Availability

Available

Length

978 bp

Topology

linear

Molecule Type

genomic DNA

promoter 4

Label	Gene	Product	Position	Strand
lac promoter	—	—	130..161	Fwd
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG
T3 promoter	—	—	229..248	Fwd
DNA Sequence AATTAACCCTCACTAAAGG
SNR52 promoter	—	—	266..535	Fwd
DNA Sequence TCTTTGAAAAGATAATGTATGATTATGCTTTCACTCATATTTATACAGAAACTTGATGTTTTCTTTCGAGTATATACAAGGTGATTACATGTACGTTTGAAGTACAACTCTAGATTTTGTAGTGCCCTCTTGGGCTAGCGGTAAAGGTGCGCATTTTTTCACACCCTACAATGTTCTGTTCAAAAGATTTTGGTCAAACGCTGTAGAAGTGAAAGTTGGTGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATC
T7 promoter	—	—	668..687	Rev
DNA Sequence TAATACGACTCACTATAGG

protein_bind 2

Label	Gene	Product	Position	Strand
CAP binding site	—	—	94..116	Fwd
DNA Sequence TAATGTGAGTTAGCTCACTCAT
lac operator	—	—	168..185	Fwd
DNA Sequence TTGTGAGCGGATAACAA

terminator 1

Label	Gene	Product	Position	Strand
SUP4 terminator	—	—	634..654	Fwd
DNA Sequence TTTTTTTGTTTTTTATGTCT