3xFLAG-dCas9/p-bacteria

64325 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.
Depositor
Hodaka Fujii
Article
Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do
Insert
3xFLAG-dCas9
Tags
3xFLAG
Mutation
D10A, H840A
Promoter
pLtetO-1
Available Since
Aug. 3, 2015
Availability
Available

Sequence Information

Length
1,062 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

terminator 1
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB — 15..62 Rev
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTAT