pJexpress414_OmpA171_H-78

64313 Synthetic Bacterial Expression

Plasmid Information

Purpose
OmpA redesign hybrid with wild-type strands 123456
Depositor
Timothy Whitehead
Article
Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p
Insert
OmpA_H-78
Tags
6His and FlAsH
Promoter
T7
Available Since
May 27, 2015
Availability
Available

Sequence Information

Length
797 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
HA — HA (human influenza hemagglutinin) epit… 523..550 Fwd
DNA Sequence
TACCCATACGATGTTCCAGATTACGCG
Protein Sequence
YPYDVPDYA
tetracysteine tag — tetracysteine peptide that binds biarse… 601..619 Fwd
DNA Sequence
TGTTGCCCTGGTTGCTGT
Protein Sequence
CCPGCC
6xHis — 6xHis affinity tag 619..637 Fwd
DNA Sequence
CACCATCACCACCATCAC
Protein Sequence
HHHHHH
sig_peptide 1
Label Gene Product Position Strand
OmpA signal peptide — signal peptide from the E. coli outer m… 13..76 Fwd
DNA Sequence
ATGAAAAAGACCGCAATCGCCATCGCAGTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCC
terminator 1
Label Gene Product Position Strand
T7 terminator — — 650..698 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG