pJexpress414_OmpA171_H346

64312 Synthetic Bacterial Expression

Plasmid Information

Purpose
OmpA redesign hybrid with wild-type strands 346
Depositor
Timothy Whitehead
Article
Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p
Insert
OmpA_H346
Tags
6His and FlAsH
Promoter
T7
Available Since
July 16, 2015
Availability
Available

Sequence Information

Length
849 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
HA HA (human influenza hemagglutinin) epit… 526..553 Fwd
DNA Sequence
TACCCATACGATGTTCCAGATTACGCG
Protein Sequence
YPYDVPDYA
tetracysteine tag tetracysteine peptide that binds biarse… 604..622 Fwd
DNA Sequence
TGTTGCCCTGGTTGCTGT
Protein Sequence
CCPGCC
6xHis 6xHis affinity tag 622..640 Fwd
DNA Sequence
CACCATCACCACCATCAC
Protein Sequence
HHHHHH
sig_peptide 1
Label Gene Product Position Strand
OmpA signal peptide signal peptide from the E. coli outer m… 16..79 Fwd
DNA Sequence
ATGAAAAAGACCGCAATCGCCATCGCAGTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCC
terminator 1
Label Gene Product Position Strand
T7 terminator 653..701 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG