pJexpress414_OmpA171_H-3

Plasmid Information

Purpose

OmpA redesign hybrid with wild-type strands 1245678

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H-3

Length

790 bp

Topology

linear

Molecule Type

genomic DNA

CDS 3

Label	Gene	Product	Position	Strand
HA	—	HA (human influenza hemagglutinin) epit…	526..553	Fwd
DNA Sequence TACCCATACGATGTTCCAGATTACGCG Protein Sequence YPYDVPDYA
tetracysteine tag	—	tetracysteine peptide that binds biarse…	604..622	Fwd
DNA Sequence TGTTGCCCTGGTTGCTGT Protein Sequence CCPGCC
6xHis	—	6xHis affinity tag	622..640	Fwd
DNA Sequence CACCATCACCACCATCAC Protein Sequence HHHHHH

sig_peptide 1

Label	Gene	Product	Position	Strand
OmpA signal peptide	—	signal peptide from the E. coli outer m…	16..79	Fwd
DNA Sequence ATGAAAAAGACCGCAATCGCCATCGCAGTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCC

terminator 1

Label	Gene	Product	Position	Strand
T7 terminator	—	—	653..701	Fwd
DNA Sequence CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG