pTPGI_dCas9 — PlasmidDB

Plasmid Information

Purpose

encodes yeast-optimized dCas9 synthetic transcription factor

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

Yeast-optimized dCas9

Length

824 bp

Topology

linear

Molecule Type

genomic DNA

CDS 1

Label	Gene	Product	Position	Strand
SV40 NLS	—	nuclear localization signal of SV40 (si…	152..173	Fwd
DNA Sequence CCAAAGAAGAAGAGAAAAGTT Protein Sequence PKKKRKV

protein_bind 1

Label	Gene	Product	Position	Strand
tet operator	tetO	—	17..36	Fwd
DNA Sequence TCCCTATCAGTGATAGAGA