punc503GFP — PlasmidDB

Plasmid Information

Purpose

503-bp promoter of zebrafish unc-45b drives muscle-specific GFP expression

Depositor

Joachim Berger, Peter Currie

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

unc-45b: GFP (unc45b Zebrafish)

Promoter

503-bp promoter of zebrafish unc-45b

Available Since

May 15, 2015

Availability

Available

Length

835 bp

Topology

linear

Molecule Type

genomic DNA

polyA_signal 2

Label	Gene	Product	Position	Strand
SV40 poly(A) signal	—	—	89..224	Fwd
DNA Sequence GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
SV40 poly(A) signal	—	—	346..481	Fwd
DNA Sequence GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT

protein_bind 2

Label	Gene	Product	Position	Strand
attB2	mutant version of attB	—	63..88	Rev
DNA Sequence CCCACTTTGTACAAGAAAGCTGGGT
attB3	mutant version of attB	—	286..307	Rev
DNA Sequence CAACTATGTATAATAAAGTTG