pDual-eGFP(Stop66)

63218 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.
Depositor
Richard S Myers
Article
Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
Insert
His-T7-eGFP(Stop66)
Tags
T7 and his6
Mutation
Changed tyrosine at position 66 with respect to t…
Promoter
CMV-EF1α hybrid (CEF)
Available Since
April 27, 2015
Availability
Available

Sequence Information

Length
639 bp
Topology
linear
Molecule Type
genomic DNA

Features

No features annotated for this plasmid.