pDual-eGFP(W66)

63217 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
Depositor
Richard S Myers
Article
Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
Insert
His-T7-eGFP(W66)
Tags
His6 and T7
Mutation
Changed tyrosine at position 66 with respect to t…
Promoter
CMV-EF1α hybrid (CEF)
Available Since
April 15, 2015
Availability
Available

Sequence Information

Length
168 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
T7 promoter — — 75..94 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 94..119 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC