pET3CTc3A(1-135)

63138 Caenorhabditis elegans Bacterial Expression

Plasmid Information

Purpose
Expression of human Tc3(1–135) bipartite domain tagged with 6xHis
Depositor
Titia Sixma
Article
Watkins et al Nucleic Acids Res. 2004 Aug 10;32(14):43
Insert
Tc3A
Tags
6xHis
Mutation
1-135 aa bipartite domain
Promoter
T7
Available Since
April 17, 2015
Availability
Available

Sequence Information

Length
766 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 415..433 Fwd
DNA Sequence
CACCATCACCATCACCAT
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 500..548 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG