pET-(pos36)GFP-9xGGS-Cre-6xHis

62938 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expression of (+36)GFP-GGS9-Cre-6xHis in bacterial cells
Depositor
David Liu
Article
Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do
Insert
(ps36)GFP-9xGGS-Cre
Tags
(pos36)GFP and 6xHis tag
Promoter
T7
Available Since
May 18, 2015
Availability
Available

Sequence Information

Length
925 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
T7 promoter — — 56..75 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 75..100 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC