pJW4.21 — PlasmidDB

Plasmid Information

Purpose

Bacterial expression of MBP-MEG-1

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MEG-1

Length

865 bp

Topology

linear

Molecule Type

genomic DNA

CDS 1

Label	Gene	Product	Position	Strand
Factor Xa site	—	Factor Xa recognition and cleavage site	33..45	Fwd
DNA Sequence ATCGAGGGAAGG Protein Sequence IEGR

protein_bind 1

Label	Gene	Product	Position	Strand
attB1	mutant version of attB	—	48..73	Fwd
DNA Sequence ACAAGTTTGTACAAAAAAGCAGGCT