pJW4.17

62872 Caenorhabditis elegans Bacterial Expression

Plasmid Information

Purpose
Bacterial expression of MBP-MBK-2 K196R
Depositor
Geraldine Seydoux
Article
Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife
Insert
MBP-MBK-2 K196R
Tags
MBP
Mutation
K196R
Available Since
March 18, 2015
Availability
Available

Sequence Information

Length
1,063 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
Factor Xa site — Factor Xa recognition and cleavage site 37..49 Fwd
DNA Sequence
ATCGAGGGAAGG
Protein Sequence
IEGR
protein_bind 1
Label Gene Product Position Strand
attB1 mutant version of attB — 52..77 Fwd
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT