pJexpress414_OmpA171_WTnoGirdles

62653 Synthetic Bacterial Expression

Plasmid Information

Purpose
Wild-type OmpA with aromatic girdle residues replaced by leucine
Depositor
Timothy Whitehead
Article
Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p
Insert
OmpA_noGirdles
Tags
6His and FlAsH
Mutation
Aromatic girdle residues mutated to Leu (W7, W15,…
Promoter
T7
Available Since
May 27, 2015
Availability
Available

Sequence Information

Length
880 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
HA — HA (human influenza hemagglutinin) epit… 549..576 Fwd
DNA Sequence
TACCCATACGATGTTCCAGATTACGCT
Protein Sequence
YPYDVPDYA
tetracysteine tag — tetracysteine peptide that binds biarse… 630..648 Fwd
DNA Sequence
TGTTGCCCTGGTTGCTGT
Protein Sequence
CCPGCC
6xHis — 6xHis affinity tag 648..666 Fwd
DNA Sequence
CACCATCACCACCATCAC
Protein Sequence
HHHHHH
sig_peptide 1
Label Gene Product Position Strand
OmpA signal peptide — signal peptide from the E. coli outer m… 27..90 Fwd
DNA Sequence
ATGAAAAAGACCGCAATCGCCATCGCAGTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCC
terminator 1
Label Gene Product Position Strand
T7 terminator — — 679..727 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG