pET-(-30)GFP-9xGGS-Cre-6xHis

62372 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells
Depositor
David Liu
Article
Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do
Insert
(-30)GFP-9xGGS-Cre-6xHis
Tags
(-30)GFP and 6xHis tag
Promoter
T7
Available Since
May 11, 2015
Availability
Available

Sequence Information

Length
908 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
T7 promoter — — 56..75 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 75..100 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC