pAN-OR-MalT-3NT

62309 Synthetic Bacterial Expression

Plasmid Information

Purpose
synthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genome
Depositor
Christopher Voigt
Article
Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:
Promoter
PA2, PPhlF, and pBAD
Available Since
April 8, 2015
Availability
Available

Sequence Information

Length
869 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
Myc — Myc (human c-Myc proto-oncogene) epitop… 144..174 Fwd
DNA Sequence
GAACAAAAACTCATCTCAGAAGAGGATCTG
Protein Sequence
EQKLISEEDL
terminator 1
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB — 429..476 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTAT