pAN-PBAD-sgRNA-scramble

62285 Synthetic Bacterial Expression

Plasmid Information

Purpose
expression of scramble sgRNA from the arabinose-inducible promoter
Depositor
Christopher Voigt
Article
Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:
Insert
scramble sgRNA
Promoter
pBAD
Available Since
April 8, 2015
Availability
Available

Sequence Information

Length
881 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
Myc — Myc (human c-Myc proto-oncogene) epitop… 147..177 Fwd
DNA Sequence
GAACAAAAACTCATCTCAGAAGAGGATCTG
Protein Sequence
EQKLISEEDL
6xHis — 6xHis affinity tag 192..210 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB — 435..482 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTAT