pAN-PBAD-sgRNA-A5T

62264 Synthetic Bacterial Expression

Plasmid Information

Purpose
expression of A5T sgRNA from the arabinose-inducible promoter
Depositor
Christopher Voigt
Article
Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:
Insert
A5T sgRNA
Promoter
pBAD
Available Since
April 8, 2015
Availability
Available

Sequence Information

Length
874 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
Myc — Myc (human c-Myc proto-oncogene) epitop… 146..176 Fwd
DNA Sequence
GAACAAAAACTCATCTCAGAAGAGGATCTG
Protein Sequence
EQKLISEEDL
6xHis — 6xHis affinity tag 191..209 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB — 434..481 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTAT