3xFLAG-dCas9/pTEF1p-CYC1t

62190 Synthetic Yeast Expression

Plasmid Information

Purpose
Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.
Depositor
Hodaka Fujii
Article
Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp
Insert
3xFLAG-dCas9
Tags
3xFLAG and NLS
Mutation
human codon-optimized, D10A, H840A
Promoter
TEF1 promoter
Available Since
Feb. 24, 2015
Availability
Available

Sequence Information

Length
1,071 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
FLAG FLAG(R) epitope tag, followed by an ent… 35..59 Fwd
DNA Sequence
GATTACAAGGATGACGATGACAAG
Protein Sequence
DYKDDDDK