pGEX6P2-p53 (1-90) (72R)

62081 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of human p53 (TAD residues 1-90) (72R) in e. coli
Depositor
Gary Daughdrill
Article
Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d
Insert
p53 (TP53 Human)
Tags
GST
Mutation
Contains TAD/PP residues 1-90, 72R
Promoter
tac
Available Since
Feb. 2, 2015
Availability
Available

Sequence Information

Length
797 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
HRV 3C site — recognition and cleavage site for human… 1..25 Fwd
DNA Sequence
CTGGAAGTTCTGTTCCAGGGGCCC
Protein Sequence
LEVLFQGP
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 640..745 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT