pET28-p53 (1-73) (K24N)

62079 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of human p53 (1-73) (K24N) in e. coli
Depositor
Gary Daughdrill
Article
Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:
Insert
p53 (TP53 Human)
Tags
His
Mutation
contains TAD residues 1-73; K24N
Promoter
T7
Available Since
Jan. 29, 2015
Availability
Available

Sequence Information

Length
732 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 179..197 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 431..449 Rev
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 458..476 Rev
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 556..575 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 531..556 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC
terminator 1
Label Gene Product Position Strand
T7 terminator — — 65..113 Rev
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG