pET28A-p53 (1-73) (PPP)

62067 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of human p53 (1-73) (P12A, P13A, P27A) in e. coli
Depositor
Gary Daughdrill
Article
Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d
Insert
p53 (TP53 Human)
Tags
His
Mutation
Contains TAD residues 1-73; P12A, P13A, P27A
Promoter
T7
Available Since
Jan. 29, 2015
Availability
Available

Sequence Information

Length
514 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
thrombin site — thrombin recognition and cleavage site 14..32 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 266..284 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 350..398 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG