pET28A-p53 (1-73) (P12A P13A)

62066 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli
Depositor
Gary Daughdrill
Article
Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d
Insert
p53 (TP53 Human)
Tags
His
Mutation
Contains TAD residues 1-73; P12A, P13A
Promoter
T7
Available Since
Feb. 23, 2015
Availability
Available

Sequence Information

Length
725 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
thrombin site — thrombin recognition and cleavage site 215..233 Rev
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 242..260 Rev
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 340..359 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 315..340 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC