pGEX6P2-MDMX (23-111)

62064 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of human MDM4 (residues 23-111) in e. coli
Depositor
Gary Daughdrill
Article
Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d
Insert
MDM4 (MDM4 Human)
Tags
GST
Mutation
contains residues 23-111
Promoter
tac
Available Since
Feb. 5, 2015
Availability
Available

Sequence Information

Length
886 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
HRV 3C site — recognition and cleavage site for human… 11..35 Fwd
DNA Sequence
CTGGAAGTTCTGTTCCAGGGGCCC
Protein Sequence
LEVLFQGP
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 632..737 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT