PlasmidDB
Home
Plasmids
Features
API
Skill
MCP
Sign in
Register
Home
Plasmids
4TVR
4TVR
61952
Homo sapiens
Bacterial Expression
Plasmid Information
Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
UBH14 (UHRF2 Human)
Tags
MHHHHHHSSGRENLYFQG
Mutation
See comments
Promoter
T7
Available Since
March 2, 2015
Availability
Available
Sequence Information
Length
616 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org
Features
CDS
2
Label
Gene
Product
Position
Strand
6xHis
—
6xHis affinity tag
22..40
Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site
—
tobacco etch virus (TEV) protease recog…
52..73
Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG