4QQD

61947 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
UHRF1:SDC130-H02:C39674 (UHRF1 Human)
Tags
6xHis and MHHHHHHSSGRENLYFQG
Mutation
See comments
Promoter
T7
Available Since
March 2, 2015
Availability
Available

Sequence Information

Length
705 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis 6xHis affinity tag 23..41 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site tobacco etch virus (TEV) protease recog… 53..74 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
6xHis 6xHis affinity tag 583..601 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH