pDEST15.1-hRPN13 (285-407) (Q337A/Q338A)

61935 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of hRPN13 (285-407) (Q337A/Q338A) in E.coli
Depositor
Chris Hill
Article
VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1
Insert
hRPN13 (285-407) (ADRM1 Human)
Tags
GST
Mutation
Construct starts at residue 285, residues Q337 an…
Promoter
T7
Available Since
Oct. 9, 2015
Availability
Available

Sequence Information

Length
790 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
TEV site — tobacco etch virus (TEV) protease recog… 465..486 Rev
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG
protein_bind 2
Label Gene Product Position Strand
attB2 mutant version of attB — 29..54 Fwd
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT
attB1 mutant version of attB — 506..531 Rev
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT