pET151-hUCH37 DM

61931 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of hUCH37 (ISF1) M269E/N291D in E.coli
Depositor
Chris Hill
Article
VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1
Insert
hUCH37 (ISF1) M269E/N291D (UCHL5 Human)
Tags
6x HIS
Mutation
Changed methionine 269 to glutamate and asparagin…
Promoter
T7
Available Since
Oct. 9, 2015
Availability
Available

Sequence Information

Length
641 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
TEV site tobacco etch virus (TEV) protease recog… 40..61 Fwd
DNA Sequence
GAAAACCTGTATTTTCAAGGA
Protein Sequence
ENLYFQG