pHOHO2_Hsp82viiA — PlasmidDB

Plasmid Information

Purpose

Yeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.

Depositor

Dan Bolon

Article

Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:

Insert

Hsp82viiA (HSP82 Budding Yeast)

Length

1,008 bp

Topology

linear

Molecule Type

genomic DNA

CDS 1

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	81..99	Fwd
DNA Sequence CACCACCATCACCACCAC Protein Sequence HHHHHH