Hs_MSI2_RBD — PlasmidDB

Plasmid Information

Purpose

Express His tagged human MSI2 RNA binding domain

Depositor

Sean Ryder

Insert

MSI2 RNA binding domain (MSI2 Human)

Length

756 bp

Topology

linear

Molecule Type

genomic DNA

CDS 2

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	457..475	Fwd
DNA Sequence CATCATCATCATCATCAC Protein Sequence HHHHHH
TEV site	—	tobacco etch virus (TEV) protease recog…	478..499	Fwd
DNA Sequence GAAAACCTGTATTTTCAGGGC Protein Sequence ENLYFQG

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	366..385	Fwd
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	385..410	Fwd
DNA Sequence GGAATTGTGAGCGGATAACAATTCC