Dm_msi_RRM1

61112 Drosophila melanogaster Bacterial Expression

Plasmid Information

Purpose
Express His tagged Drosophila musashi RRM1
Depositor
Sean Ryder
Insert
Drosophila musashi RBD
Tags
6xHis-TEV
Mutation
stop codon after first RRM domain
Available Since
Jan. 15, 2015
Availability
Available

Sequence Information

Length
770 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 466..484 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 487..508 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG
promoter 1
Label Gene Product Position Strand
T7 promoter — — 375..394 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 394..419 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC