Dm_msi_T

61110 Drosophila melanogaster Bacterial Expression

Plasmid Information

Purpose
Express His tagged Drosophila musashi RNA binding domain
Depositor
Sean Ryder
Insert
musashi RNA binding domain
Tags
6xHis-TEV
Available Since
Jan. 15, 2015
Availability
Available

Sequence Information

Length
752 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 461..479 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 482..503 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG
promoter 1
Label Gene Product Position Strand
T7 promoter — — 370..389 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 389..414 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC