SENP2M497A

61088 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
cleave the SUMO off when use PETSUMO vector for express fusion protein
Depositor
Cynthia Wolberger
Article
Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033
Insert
SENP2M497A catalytic domain (SENP2 Human)
Tags
His
Mutation
M497A; Catalytic domain is residues 364-589
Promoter
T7
Available Since
Jan. 29, 2015
Availability
Available

Sequence Information

Length
663 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 142..160 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 169..187 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 43..62 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 62..87 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC