Purpose
Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.
Depositor
Nils Walter
Article
Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145
Insert
Cwc25
Tags
TEV site, Histidine Tag
Promoter
T7
Available Since
Jan. 27, 2015
Availability
Available