pCRII HSPB15

61039 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Probe for HSPB51b/15 for in situ hybridization in zebrafish
Depositor
Lara Hutson
Article
Marvin et al Dev Dyn. 2008 Feb;237(2):454-63.
Insert
HSPB15 (hspb15 Zebrafish)
Available Since
Dec. 16, 2014
Availability
Available

Sequence Information

Length
1,030 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
Factor Xa site Factor Xa recognition and cleavage site 652..664 Fwd
DNA Sequence
ATCGAAGGGCGA
Protein Sequence
IEGR
promoter 2
Label Gene Product Position Strand
SP6 promoter 21..40 Fwd
DNA Sequence
ATTTAGGTGACACTATAGA
T7 promoter 726..745 Rev
DNA Sequence
TAATACGACTCACTATAGG