pET28a-HIS-Xyl-Xmod-DocIII

60865 Synthetic Bacterial Expression

Plasmid Information

Purpose
Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C
Depositor
Hermann Gaub
Article
Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1
Tags
6xHIS
Mutation
Threonine 120 to Cysteine in Xylanase domain
Promoter
T7
Available Since
Nov. 26, 2014
Availability
Available

Sequence Information

Length
766 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 36..54 Fwd
DNA Sequence
CACCATCACCATCACCAT
Protein Sequence
HHHHHH