pESC-URA-hPOLISc

60819 Homo sapiens Yeast Expression

Plasmid Information

Purpose
to express human Pol iota recoded to yeast codons in yeast
Depositor
Youri Pavlov
Article
Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:
Insert
POLI (POLI Human)
Tags
c-myc
Mutation
optimized to yeast codon usage
Promoter
GAL1
Available Since
March 9, 2015
Availability
Available

Sequence Information

Length
947 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
Myc — Myc (human c-Myc proto-oncogene) epitop… 38..68 Fwd
DNA Sequence
GAACAGAAGTTGATTTCCGAAGAAGACCTC
Protein Sequence
EQKLISEEDL
promoter 1
Label Gene Product Position Strand
T7 promoter — — 2..21 Fwd
DNA Sequence
TAATACGACTCACTATAGG