pYIAL31 — PlasmidDB

Plasmid Information

Purpose

used to make mutations in yeast POL1

Depositor

Thomas Kunkel, Youri Pavlov

Article

Pavlov et al Genetics. 2001 Sep;159(1):47-64.

Insert

Pol1 (POL1 Budding Yeast)

Mutation

N-terminal part only

Available Since

Feb. 6, 2015

Availability

Available

Length

933 bp

Topology

linear

Molecule Type

genomic DNA

misc_feature 1

Label	Gene	Product	Position	Strand
MCS	—	—	298..355	Fwd
DNA Sequence GAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTT

promoter 1

Label	Gene	Product	Position	Strand
lac promoter	—	—	211..242	Fwd
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG

protein_bind 2

Label	Gene	Product	Position	Strand
CAP binding site	—	—	175..197	Fwd
DNA Sequence TAATGTGAGTTAGCTCACTCAT
lac operator	—	—	249..266	Fwd
DNA Sequence TTGTGAGCGGATAACAA